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网页Pour each standard solution into labeled small vials in a sample rack. Store the racks of samples in a refrigerator, along with the remaining solutions in the 50-mL volumetric flasks. 4. Checking the Initial Settings of the HPLC
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网页For this, separate injections of blank, standard, and sample solutions into the HPLC apparatus were made. The overlay chromatogram result is displayed in. RMV was eluted at about 4.01 minutes under the ( Table 4 ) chosen chromatographic conditions (Blank Solution- and Standard RMV solution).
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网页2023年1月26日 · An aliquot of 10 μL plasma was pipetted on the Whatman 903® filter paper, and kept drying for at least 2 hours at room temperature to prepare as DPS samples. For subsequent analysis, the disc (13 mm diameter, the entire DPS) was punched from each filter card by a holing puncher and then was transferred to a tube.